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Promega pgem7 vector
Pgem7 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem7+-zf(+)+vector/pgem+t+easy/us11154611-708-12-14
Average 90 stars, based on 1 article reviews
pgem7 vector - by Bioz Stars, 2026-10
90/100 stars

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Amplification:

Article Title: The Bdellovibrio bacteriovorus twin-arginine transport system has roles in predatory and prey-independent growth.
Article Snippet: .. Each was amplified including flanking DNA, from genomic DNA of B. bacteriovorus HD100 using primers listed in Supplementary Table S2 and Phusion High-Fidelity DNA polymerase (New England Biolabs), and cloned into the EcoRI site of the cloning vectors pGEM7 (Promega; tatA1 and tatA2) or pUC19 (Vieira & Messing, 1982; tatC). ..

Clone Assay:

Article Title: The Bdellovibrio bacteriovorus twin-arginine transport system has roles in predatory and prey-independent growth.
Article Snippet: .. Each was amplified including flanking DNA, from genomic DNA of B. bacteriovorus HD100 using primers listed in Supplementary Table S2 and Phusion High-Fidelity DNA polymerase (New England Biolabs), and cloned into the EcoRI site of the cloning vectors pGEM7 (Promega; tatA1 and tatA2) or pUC19 (Vieira & Messing, 1982; tatC). ..

Article Title: Sequential monitoring of transgene expression following Agrobacterium-mediated transformation of rice.
Article Snippet: For the fluc expression vector, pCAMBIA1390-sGFP (Fig. 2A; Niwa et al. 1999, Niwa 2003, Toki et al. 2006) was digested with SalI and BsrGI [bluntended with T4 DNA polymerase (Toyobo)], and the SalI-/SacIdigested (blunt-ended with T4 DNA polymerase) fluc fragment from the fluc expression vector (Promega) was inserted. .. For the eluc expression vector, the sgfp expression cassette was cloned into the vector pGEM7 (Promega) using HindIII and EcoRI. .. The sgfp fragment was replaced with the eluc fragment derived from the pELuc test vector (Toyobo) by NcoI and NotI digestion.

Cloning:

Article Title: The Bdellovibrio bacteriovorus twin-arginine transport system has roles in predatory and prey-independent growth.
Article Snippet: .. Each was amplified including flanking DNA, from genomic DNA of B. bacteriovorus HD100 using primers listed in Supplementary Table S2 and Phusion High-Fidelity DNA polymerase (New England Biolabs), and cloned into the EcoRI site of the cloning vectors pGEM7 (Promega; tatA1 and tatA2) or pUC19 (Vieira & Messing, 1982; tatC). ..

Plasmid Preparation:

Article Title: Vaccine against bovine leukemia virus
Article Snippet: This insert was digested with EcoRI and XbaI and inserted into the pGEM7 vector (Promega) yielding pGEMXR3. .. This insert was digested with EcoRI and XbaI and inserted into the pGEM7 vector (Promega) yielding pGEMXR3. ..

Article Title: Transgenic proteins from multi-gene systems, methods, compositions, uses and the like relating thereto
Article Snippet: .. Production of the Plasmid pXKI The plasmid pXKI was assembled from the coding segment of factor X light chain (first 179 amino acids) and the Kunitz domain 1 (KI) coding segment of Tissue Factor Plasminogen Inhibitor (TFPI) and placed in the vector pGEM7 (Promega; Madison, Wis.), (FIG. 1). ..

Article Title: Identification of Light-Sensitive Phosphorylation Sites on PERIOD That Regulate the Pace of Circadian Rhythms in Drosophila
Article Snippet: .. An XbaI-BamHI subfragment of this vector including sequences encoding amino acids (aa) 1 to 870 of dPER was subcloned into the pGEM7 vector (Promega), and the resulting plasmid was used as the template for site-directed mutagenesis using a QuikChange site-directed mutagenesis kit (Agilent). ..

Article Title: G6PC2 Modulates the Effects of Dexamethasone on Fasting Blood Glucose and Glucose Tolerance
Article Snippet: .. PCR fragments were digested with Hin dIII and Xho I and ligated into the pGEM7 vector (Promega) for DNA sequencing. ..

Article Title: Sequential monitoring of transgene expression following Agrobacterium-mediated transformation of rice.
Article Snippet: For the fluc expression vector, pCAMBIA1390-sGFP (Fig. 2A; Niwa et al. 1999, Niwa 2003, Toki et al. 2006) was digested with SalI and BsrGI [bluntended with T4 DNA polymerase (Toyobo)], and the SalI-/SacIdigested (blunt-ended with T4 DNA polymerase) fluc fragment from the fluc expression vector (Promega) was inserted. .. For the eluc expression vector, the sgfp expression cassette was cloned into the vector pGEM7 (Promega) using HindIII and EcoRI. .. The sgfp fragment was replaced with the eluc fragment derived from the pELuc test vector (Toyobo) by NcoI and NotI digestion.

Mutagenesis:

Article Title: Identification of Light-Sensitive Phosphorylation Sites on PERIOD That Regulate the Pace of Circadian Rhythms in Drosophila
Article Snippet: .. An XbaI-BamHI subfragment of this vector including sequences encoding amino acids (aa) 1 to 870 of dPER was subcloned into the pGEM7 vector (Promega), and the resulting plasmid was used as the template for site-directed mutagenesis using a QuikChange site-directed mutagenesis kit (Agilent). ..

Polymerase Chain Reaction:

Article Title: G6PC2 Modulates the Effects of Dexamethasone on Fasting Blood Glucose and Glucose Tolerance
Article Snippet: .. PCR fragments were digested with Hin dIII and Xho I and ligated into the pGEM7 vector (Promega) for DNA sequencing. ..

DNA Sequencing:

Article Title: G6PC2 Modulates the Effects of Dexamethasone on Fasting Blood Glucose and Glucose Tolerance
Article Snippet: .. PCR fragments were digested with Hin dIII and Xho I and ligated into the pGEM7 vector (Promega) for DNA sequencing. ..

other:

Article Title: Identification of Light-Sensitive Phosphorylation Sites on PERIOD That Regulate the Pace of Circadian Rhythms in Drosophila
Article Snippet: A XbaI–BamHI subfragment of this vector 117 including sequences encoding amino acids 1–870 of dPER was subcloned into pGEM7 118 vector (Promega); and the resulting plasmid was used as the template for site-directed 119 mutagenesis using the QuickChange Site-Directed Mutagenesis kit (Agilent).

Expressing:

Article Title: Sequential monitoring of transgene expression following Agrobacterium-mediated transformation of rice.
Article Snippet: For the fluc expression vector, pCAMBIA1390-sGFP (Fig. 2A; Niwa et al. 1999, Niwa 2003, Toki et al. 2006) was digested with SalI and BsrGI [bluntended with T4 DNA polymerase (Toyobo)], and the SalI-/SacIdigested (blunt-ended with T4 DNA polymerase) fluc fragment from the fluc expression vector (Promega) was inserted. .. For the eluc expression vector, the sgfp expression cassette was cloned into the vector pGEM7 (Promega) using HindIII and EcoRI. .. The sgfp fragment was replaced with the eluc fragment derived from the pELuc test vector (Toyobo) by NcoI and NotI digestion.



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